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ATTO 647 DBCO,ATTO 647二苯并环辛炔,DBCO试剂可以通过无铜点击化学与叠氮反应

发布时间:2023-10-18 分享至:

  产品名称:ATTO 647 DBCO

  AT 647 DBCO

  中文名称:AT 647二苯并环辛炔

  化学式:C52H59N4O5S+

  分子量:852.13

  纯度:95%

  保存条件:避光,干燥,-20℃保存

  注意事项:取用一定要干燥,避免频繁的溶解和冻干。

  用途:仅用于科学研究或者工业应用等非医疗目的,非药用,非食用

  ATTO 647 belongs to a new generation of fluorescent labels for the red spectral region. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photo-stability, and good water solubility. ATTO 647 is a zwitterionic dye with a net electrical charge of zero. The dye is stable at physiological pH-values and in buffers up to pH 8. However, it slowly degrades at higher pH. The dye is very hydrophilic. The fluorescence is excited most efficiently in the range 615 - 660 nm. A suitable excitation source for ATTO 647 is, next to the He:Ne laser (633nm), the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.

  DBCO reagent is a class of click chemistry labeling reagents containing a very reactive DBCO ((Dibenzocyclooctyne) group, DBCO reagent can react with azide-tagged molecules or biomolecules via copper-free Click Chemistry. DBCO click chemistry can be run in aqueous buffer or in organic solvents depending on the property of the substrate molecules. Reagents with PEG arm will increases the compound's hydrophilicity.

  DBCO试剂是一类含有非常活泼的DBCO(二苯并环辛炔)基团的点击化学标记试剂,DBCO试剂可以通过无铜点击化学与叠氮标记分子或生物分子反应。DBCO点击化学可以在水缓冲液或有机溶剂中运行,具体取决于底物分子的性质。带有PEG臂的试剂将增加化合物的亲水性。

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