5-TAMRA-NHS ester,150810-68-7,5-羧基-四甲基罗丹明-琥珀酰亚胺酯 的应用分享
发布时间:2023-10-27 分享至:
英文名称:5-TAMRA-NHS ester
5-TAMRA-SE
中文名称:5-羧基-四甲基罗丹明-琥珀酰亚胺酯
CAS:150810-68-7
Abs/Em值:553/575纳米
消光系数:92,000 cm-1M-1
溶解度:二甲基亚砜,二甲基亚砜,甲醇
光谱相似染料:Alexa Fluor 546
分子量:527.53
储存条件:-20℃
运输:低温运输
尽管 5(6)-TAMRA Acid的混合异构体是一种的、常规用于蛋白质染色的橙色荧光染料,但它***终用于标记肽和核苷酸。由于高效液相色谱纯化过程中信号明显展宽,5(6)-TAMRA标记的肽和核苷酸的纯化可能比较麻烦。用单一异构体TAMRA标记的肽和核苷酸通常在接合过程中经常需要的HPLC纯化中提供更好的分辨率。5-TAMRA更常用于标记肽和蛋白质。6-TAMRA主要用于标记核苷酸和核酸测序。5-TAMRA染料是一种明亮的荧光标签,与各种激发源兼容,包括汞弧灯、钨弧灯和氙弧灯、544 nm氦氖激光线和532 nm绿色激光线。
NHS酯在pH 7-9下与伯胺(例如赖氨酸残基侧链或氨基硅烷涂层表面)特异且有效地反应,以形成稳定的共价酰胺键。NHS酯(或琥珀酰亚胺酯)是将染料与蛋白质或抗体的伯胺(Lys)、胺修饰的寡核苷酸和其他含胺分子结合的常用工具。
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